Hydrolysis
Hydrolysis usually means the rupture of chemical bonds by the addition of water. Generally, hydrolysis is a step in the degradation of a substance. In terms of the word's derivation, hydrolysis (11px /haɪˈdrɒlɨsɪs/) comes from Greek roots hydro "water" + lysis "separation".
Contents
Types
Usually hydrolysis is a chemical process in which a molecule of water molecule adds to a substance. Sometimes this addition causes the substance to split into two parts. In such reactions, one fragment of the target molecule (or parent molecule) gains a hydrogen ion (H+) from the split water molecule. The other portion of the target molecule collects the hydroxyl group (OH−) of the split water molecule. In effect an acid and a base are formed.
Salts
A common kind of hydrolysis occurs when a salt of a weak acid or weak base (or both) is dissolved in water. Water spontaneously ionizes into hydroxyl anions and hydrogen cations. The salt, too, dissociates into its constituent anions and cations. For example, sodium acetate dissociates in water into sodium and acetate ions. Sodium ions react very little with the hydroxyl ions whereas the acetate ions combine with hydrogen ions to produce acetic acid. In this case the net result is a relative excess of hydroxyl ions, giving a basic solution.
Strong acids also undergo hydrolysis. For example, dissolving sulfuric acid in water is accompanied by hydrolysis to give hydronium and bisulfate.
Esters and amides
Acid–base-catalysed hydrolyses are very common; one example is the hydrolysis of amides or esters. Their hydrolysis occurs when the nucleophile (a nucleus-seeking agent, e.g., water or hydroxyl ion) attacks the carbon of the carbonyl group of the ester or amide. In an aqueous base, hydroxyl ions are better nucleophiles than polar molecules such as water. In acids, the carbonyl group becomes protonated, and this leads to a much easier nucleophilic attack. The products for both hydrolyses are compounds with carboxylic acid groups.
Perhaps the oldest commercially practiced example of ester hydrolysis is saponification (formation of soap). It is the hydrolysis of a triglyceride (fat) with an aqueous base such as sodium hydroxide (NaOH). During the process, glycerol is formed, and the fatty acids react with the base, converting them to salts. These salts are called soaps, commonly used in households.
In addition, in living systems, most biochemical reactions (including ATP hydrolysis) take place during the catalysis of enzymes. The catalytic action of enzymes allows the hydrolysis of proteins, fats, oils, and carbohydrates. As an example, one may consider proteases (enzymes that aid digestion by causing hydrolysis of peptide bonds in proteins). They catalyse the hydrolysis of interior peptide bonds in peptide chains, as opposed to exopeptidases (another class of enzymes, that catalyse the hydrolysis of terminal peptide bonds, liberating one free amino acid at a time).
However, proteases do not catalyse the hydrolysis of all kinds of proteins. Their action is stereo-selective: Only proteins with a certain tertiary structure will be targeted. As some kind of orienting force is needed to place the amide group in the proper position for catalysis. The necessary contacts between an enzyme and its substrates (proteins) are created because the enzyme folds in such a way as to form a crevice into which the substrate fits; the crevice also contains the catalytic groups. Therefore, proteins that do not fit into the crevice will not undergo hydrolysis. This specificity preserves the integrity of other proteins such as hormones, and therefore the biological system continues to function normally.
Upon hydrolysis, an amide converts into a carboxylic acid and an amine or ammonia. The carboxylic acid has a hydroxyl group derived from a water molecule and the amine (or ammonia) gains the hydrogen ion. The hydrolysis of peptides gives amino acids.
Many polyamide polymers such as nylon 6,6 hydrolyse in the presence of strong acids. The process leads to depolymerization. For this reason nylon products fail by fracturing when exposed to small amounts of acidic water. Polyesters are also susceptible to similar polymer degradation reactions. The problem is known as stress corrosion cracking.
ATP
Hydrolysis is related to energy metabolism and storage. All living cells require a continual supply of energy for two main purposes: for the biosynthesis of micro and macromolecules, and for the active transport of ions and molecules across cell membranes. The energy derived from the oxidation of nutrients is not used directly but, by means of a complex and long sequence of reactions, it is channelled into a special energy-storage molecule, adenosine triphosphate (ATP). The ATP molecule contains pyrophosphate linkages (bonds formed when two phosphate units are combined together) that release energy when needed. ATP can undergo hydrolysis in two ways: the removal of terminal phosphate to form adenosine diphosphate (ADP) and inorganic phosphate, or the removal of a terminal diphosphate to yield adenosine monophosphate (AMP) and pyrophosphate. The latter usually undergoes further cleavage into its two constituent phosphates. This results in biosynthesis reactions, which usually occur in chains, that can be driven in the direction of synthesis when the phosphate bonds have undergone hydrolysis.
Hydrolysis of polysaccharides
Monosaccharides can be linked together by glycosidic bonds, which can be cleaved by hydrolysis. Two, three, several or many monosaccharides thus linked form disaccharides, trisaccharides, oligosaccharides or polysaccharides, respectively. Enzymes that hydrolyse glycosidic bonds are called "glycoside hydrolases" or "glycosidases".
The best-known disaccharide is sucrose (table sugar). Hydrolysis of sucrose yields glucose and fructose. Invertase is a sucrase used industrially for the hydrolysis of sucrose to so-called invert sugar. Lactase is essential for digestive hydrolysis of lactose in milk. Deficiency of lactase in humans causes lactose intolerance.
The hydrolysis of polysaccharides to soluble sugars is called "saccharification". Malt made from barley is used as a source of β-amylase to break down starch into the disaccharide maltose, which can be used by yeast to produce beer. Other amylase enzymes may convert starch to glucose or to oligosaccharides. Cellulose is converted to glucose or the disaccharide cellobiose by cellulases. Animals such as cows (ruminants) are able to digest cellulose because of symbiotic bacteria that produce cellulases.
Hydrolysis of metal aqua ions
Metal ions are Lewis acids, and in aqueous solution they form aqua ions of the general formula M(H2O)nm+.[1][2] The aqua ions undergo hydrolysis, to a greater or lesser extent. The first hydrolysis step is given generically as
- M(H2O)nm+ + H2O 15px M(H2O)n-1(OH)(m-1)+ + H3O+
Thus the aqua cations behave as acids in terms of Brønsted-Lowry acid-base theory. This effect is easily explained by considering the inductive effect of the positively charged metal ion, which weakens the O-H bond of an attached water molecule, making the liberation of a proton relatively easy.
The dissociation constant, pKa, for this reaction is more or less linearly related to the charge-to-size ratio of the metal ion.[3] Ions with low charges, such as Na+ are very weak acids with almost imperceptible hydrolysis. Large divalent ions such as Ca2+, Zn2+, Sn2+ and Pb2+ have a pKa of 6 or more and would not normally be classed as acids, but small divalent ions such as Be2+ undergo extensive hydrolysis. Trivalent ions like Al3+ and Fe3+ are weak acids whose pKa is comparable to that of acetic acid. Solutions of salts such as BeCl2 or Al(NO3)3 in water are noticeably acidic; the hydrolysis can be suppressed by adding an acid such as nitric acid, making the solution more acidic.
Hydrolysis may proceed beyond the first step, often with the formation of polynuclear species via the process of olation.[3] Some "exotic" species such as Sn3(OH)42+ [4] are well characterized. The rate of hydrolysis tends to increase as pH rises leading, in many cases, to the precipitation of a hydroxide such as Al(OH)3 or AlO(OH). These substances, the major constituents of bauxite, are known as laterites and are formed by leeching from rocks of most of the ions other than aluminium and iron and subsequent hydrolysis of the remaining aluminium and iron.
See also
- Adenosine triphosphate
- Catabolism
- Condensation reaction
- Dehydration synthesis
- Hydrolysis constant
- Inhibitor protein
- Polymer degradation
- Solvolysis
- Thermal hydrolysis
- Tissue Digestion
References
